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jove.com
Video: Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy
8.8K Views. Reference and Translation Center for Cardiac Stem Cell Therapy (RTC). Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the ...
9.1K views
Jun 19, 2017
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#Fluorescence recovery after photobleaching FRAP Applications
SOLVED: The lateral diffusion of plasma membrane proteins has been studied using the fluorescence recovery after photobleaching (FRAP) technique in cells under normal conditions. The following plot has been obtained under different conditions (e.g., when the signaling pathway is turned on). The protein binds to a few other proteins and protein complexes in the plasma membrane, resulting in a reduced lateral diffusion coefficient. Additionally, a significant fraction of the molecules of this prot
numerade.com
Jul 19, 2022
Fluorescence Recovery After Photobleaching (FRAP) on Make a GIF
makeagif.com
Apr 23, 2022
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