PPT - Polyacrylamide gel electrophoresis (PAGE) PowerPoint Presentation - ID:7078688
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SOLVED: Investigators decide to analyze the purity of a preparation of antibody molecules using SDS polyacrylamide-gel electrophoresis (SDS-PAGE). In Lane 1 of the gel, they load a sample of the antibody. In Lane 2, they load an antibody sample that has been treated with a reducing agent called mercaptoethanol, which breaks disulfide linkages. Following electrophoresis, they see distinct bands representing polypeptides with molecular weights of 50 kD and 25 kD in Lane 2, and only one band weighi
SOLVED: Investigators decide to analyze the purity of a preparatio…
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