In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

TIRF is a microscopy technique that is used to image fluorescent molecules, such as green fluorescent protein (GFP) and fluorochromes, in liquids that are adjacent to a solid with a high refractive ...

Refraction close refractionProcess by which a wave changes speed and sometimes direction upon entering a denser or less dense medium, eg a light ray changes direction when refracted by a lens. from a ...

The body hairs of the Saharan silver ant cause total internal reflection of light to make the ants almost ten times more reflective, preventing overheating and yielding their silver sheen, according ...

Cool operator: the Saharan silver ant Cataglyphis bombycina. (Courtesy: P Landmann) A species of desert-dwelling ant is able to tolerate temperatures exceeding 50 °C because its body hairs act like ...

Bound states in the continuum (BICs) are a special class of resonant states. Although they have the same momentum and energy as the propagating modes in free space, yet they are not coupled to the ...

The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...