Reversible phosphorylation is a key signaling mechanism for modulating the functional properties of proteins involved in gene expression, cell adhesion, cell cycle, cell proliferation or cell ...
Electrophoresis is a major technique for separating proteins and other substances such as nucleic acids, purines, pyrimidines, some organic compounds, and even inorganic ions. Most of the current ...
Since the 18th-century researchers have studied proteins 1, but a number of discoveries in the 1960s that demonstrated sodium dodecyl sulfate (SDS) could facilitate the separation of proteins, ushered ...
Quality control of proteins is essential to ensure the integrity of samples before their use in assays, experiments or product release. Traditional sodium dodecyl sulfate-polyacrylamide gel ...
To achieve clear and accurate resolution of protein bands in protein gel electrophoresis, it is important to use the appropriate sample preparation conditions and to choose the right gel chemistry for ...
We tested the hypothesis that depletion of intracellular glutathione (GSH) during heat shock results in protein thiol oxidation, thereby increasing thermal sensitivity. Depletion of GSH was ...
Figure 1: Separation of dodecylmaltoside-solubilized mitochondrial complexes exemplifying the native mass range (<100 kDa to ∼10 MDa) covered by BN-PAGE. Following solubilization of biological ...
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