Most sporadic solid tumors require several mutational genetic events prior to acquisition of a malignant phenotype and development of neoplasia. Age-specific incidence data in head and neck squamous ...

To validate our technique, we selected alveolar macrophages as target cells, which contain transcriptionally inducible genes and may—apart from cell picking—be alternatively acquired from the same ...

Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

Diagnosis of Rickettsia infection would benefit by use of the more rapid and sensitive method of quantitative realtime PCR than the time-intensive and less sensitive method of culturing Rickettsia ...

The polymerase chain reaction (PCR), invented about three decades ago, soon entered mainstream use thanks to an ongoing series of refinements. One particularly important refinement, introduced about ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

In 2011, the European Court of Justice decided upon the need to demonstrate that pollen is a natural constituent of honey rather than an ingredient. Later, in 2013, the European Parliament defined ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale ...

The biolistic method is reliable for delivering genes of interest into various species, but low transformation efficiency can be a limiting factor in its application. To test various conditions that ...