Most sporadic solid tumors require several mutational genetic events prior to acquisition of a malignant phenotype and development of neoplasia. Age-specific incidence data in head and neck squamous ...

To validate our technique, we selected alveolar macrophages as target cells, which contain transcriptionally inducible genes and may—apart from cell picking—be alternatively acquired from the same ...

Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

Diagnosis of Rickettsia infection would benefit by use of the more rapid and sensitive method of quantitative realtime PCR than the time-intensive and less sensitive method of culturing Rickettsia ...

The polymerase chain reaction (PCR), invented about three decades ago, soon entered mainstream use thanks to an ongoing series of refinements. One particularly important refinement, introduced about ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

The biolistic method is reliable for delivering genes of interest into various species, but low transformation efficiency can be a limiting factor in its application. To test various conditions that ...

• A real-time PCR protocol based on 18S rDNA sequences was developed to provide a specific, sensitive and quantitative assay for the root-infecting virus vector Polymyxa graminis. • The assay was ...