Ten accessions belonging to the Brassica oleracea subspecies alba and rubra, and to B. oleracea var. sabauda were used in this study. Protoplasts were isolated from leaves and hypocotyls of in vitro ...
A method was developed for culturing protoplasts freshly isolated from developing soybean (Glycine max L.) cotyledons. First cell divisions were observed within 5 days after protoplast isolation and ...
Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by sequencing, ...
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