Pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- ...

PCR-based methods offer advantages over more traditional diagnostic tests, in that organisms do not need to be cultured prior to their detection and protocols are highly sensitive and rapid.

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

Gene targeting, the use of homologous recombination in mouse embryonic stem (ES) cells to modify mouse genes precisely 1,2,3,4,5, allows researchers to create virtually any desired modification in the ...

My cry of frustration echoed down the hall. My latest attempt to subclone five important PCR products had failed yet again. After two months of fruitless effort, I was at my wit's end until a ...

Colony picking is a very labor-intensive task that is generally performed during cloning protocols, alongside additional upstream and downstream processes. Manual colony picking is both slow and ...